Semen samples were analyzed for sperm concentration, motility, and motion parameters using a computer-aided semen analyzer (CASA; version 10HTM-IVOS; Hamilton-Thorne Research, Beverly, MA) as previously described ( Meeker et al. 2004a , 2008 ). Total sperm count (10 6 per ejaculate) was calculated by multiplying sperm concentration (10 6 /mL) by semen sample volume (milliliters). Motile sperm was defined as World Health Organization (WHO) grade “a” sperm (rapidly progressive with a velocity ≥ 25 μm/sec at 37°C) plus “b” grade sperm (slow/sluggish progressive with a velocity of ≥ 5 μm/sec but < 25 μm/sec) (WHO 1999). Of seven CASA motion variables measured, we included only three in our analysis [straight-line velocity (VSL), curvilinear velocity (VCL), and linearity (VSL/VCL × 100)] because of a high degree of dependence between several of the measures ( Duty et al. 2004 ; Meeker et al. 2004a ). Sperm morphology was assessed on two slides per specimen (with a minimum of 200 cells assessed per slide) with a Nikon microscope using an oil-immersion 100× objective (Nikon Company, Tokyo, Japan). We used strict Kruger scoring criteria to classify men as having normal or below normal morphology ( Kruger et al. 1988 ).
There is considerable controversy over the earliest age at which it is clinically, morally, and legally safe to use GnRH analogues, and for how long. The sixth edition of the World Professional Association for Transgender Health 's Standards of Care permit it from Tanner stage 2 but do not allow the addition of hormones until age 16, which could be five or more years later. Sex steroids have important functions in addition to their role in puberty, and some skeletal changes (such as increased height) that may be considered masculine are not hindered by GnRH analogues.
Walk 30 minutes daily. High Estrogen Levels are modulated by cruciferous vegetables, such as kale, collard greens, mustard greens, broccoli, cabbage and turnips. Eat one serving daily. A good source of Natural Calcium which is easily assimilated in the body is Sesame seeds. The Black variety has a higher therapeutic value and lower Oil content. You may soak overnight and eat. Chew to a fine paste. Include Sesame Seeds with your daily meals. They are a rich source of Calcium, Proteins, Magnesium and Iron. Eat Tahini (white Sesame Seed Paste) / white Sesame Seed + Dried Roasted Coconut Chutney with every meal. Fresh Coconut water and Carrot / Orange Juice are also rich sources of Calcium. Almonds are another rich source of Calcium. They contain trace mineral Boron which is essential for proper assimilation of Calcium in the body. Soak 5 - 10 Almonds overnight, skin and eat every morning.